Lipase A (gene lipA) from Bacillus subtilis

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 181 amino acids

AEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKALPGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN

Manfred T. Reetz et al. (2010) — Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

Chemical Communications · doi:10.1039/c0cc02657c ↗ · Activity - Classical Stability - Half-life

105 measurements ▼

Database ID

UniProt: P37957 ↗

Sequence Annotation

Inferred - from protein (31 first residues from Uniprot sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (105 measurements)

105 measurements — page 1 of 6

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	—	63	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	—	17	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	—	75	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	—	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	—	56	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	—	54	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	—	50	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	—	75	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	—	70	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	—	64	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	—	62	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	—	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	66	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	62	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	55	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	50	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	—	63	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	—	17	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	—	0	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	—	75	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	—	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	—	56	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	—	54	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	—	50	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	—	75	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	—	70	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	—	64	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	—	62	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	—	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	66	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	62	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	58	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	55	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	50	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	80	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	77	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	62	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	60	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	59	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	92	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	81	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	80	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	80	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	76	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	94	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	90	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	85	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	82	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	81	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100	63	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100	17	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100	0	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100	0	96	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100	0	88	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	88	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	58	78	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	76	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	54	74	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	50	70	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	89	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	58	80	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	80	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	54	76	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	50	73	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	92	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	58	88	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	84	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	54	82	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	50	78	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	94	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	58	92	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	90	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	54	85	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	50	81	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	75	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	58	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	54	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	50	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	75	84	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	70	78	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	64	76	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	62	74	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	58	68	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	75	85	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	70	79	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	64	77	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	62	76	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	58	70	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	75	94	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	70	90	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	64	88	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	62	86	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	58	82	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	75	96	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	70	95	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	64	92	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	62	88	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	58	84	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	75	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	70	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	64	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	62	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
R33G/K112D/M134D/Y139C/I157M	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	58	100	%	?	?	30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
M134D	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Acetonitrile	50%	—	12	214	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Acetonitrile	50%	—	12	352	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Acetonitrile	50%	—	12	541	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Acetonitrile	50%	—	12	994	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
R33G/K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Acetonitrile	50%	—	12	1662	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethyl Sulfoxide (DMSO)	50%	—	12	30	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethyl Sulfoxide (DMSO)	50%	—	12	49	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethyl Sulfoxide (DMSO)	50%	—	12	128	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethyl Sulfoxide (DMSO)	50%	—	12	132	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
R33G/K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethyl Sulfoxide (DMSO)	50%	—	12	220	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethylformamide (DMF)	50%	—	6	27	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethylformamide (DMF)	50%	—	6	31	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethylformamide (DMF)	50%	—	6	129	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethylformamide (DMF)	50%	—	6	135	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)
R33G/K112D/M134D/Y139C/I157M	Stability - Half-life	Half-life (in hours) in organic solvent, with remaining activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement)	Dimethylformamide (DMF)	50%	—	6	191	hours	?	?	Incubation: 30°C, Assay: 30°C	? mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in hours)

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Mutations in this dataset (6) — Manfred T. Reetz et al. (2010)

WT R33G/K112D/M134D/Y139C/I157M K112D/M134D/Y139C/I157M M134D M134D/I157M M134D/Y139C/I157M

Visualization : Activity — Classical

Manfred T. Reetz et al. (2010)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Stability — Half-life

Manfred T. Reetz et al. (2010)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Md. Zahid Kamal et al. (2013) — Lipase in aqueous-polar organic solvents: Activity, structure, and stability

Protein Science · doi:10.1002/pro.2271 ↗ · Activity - Classical Activity - Michaelis-Menten Stability - Tm

124 measurements ▼

Database ID

UniProt: P37957 ↗

Sequence Annotation

Inferred - from protein name (31 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli

Experimental Data (124 measurements)

124 measurements — page 6 of 7

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	10%	78.0	—	69.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	20%	78.0	—	61.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	30%	78.0	—	57.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	40%	78.0	—	56.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	50%	78.0	—	57.5	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	60%	78.0	—	58.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	78.0	—	76.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	78.0	—	76.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	78.0	—	78.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	78.0	—	80.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethylformamide (DMF)	10%	78.0	—	71.4	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethylformamide (DMF)	20%	78.0	—	69.6	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	10%	78.0	—	70.5	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	20%	78.0	—	65.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	30%	78.0	—	64.6	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	40%	78.0	—	64.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	50%	78.0	—	65.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	60%	78.0	—	66.0	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	10%	78.0	—	72.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	20%	78.0	—	68.3	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)

WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	5%	100.0	—	88.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100.0	—	58.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	15%	100.0	—	33.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100.0	—	16.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	25%	100.0	—	10.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	5%	100.0	—	102.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	100.0	—	107.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	15%	100.0	—	91.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	20%	100.0	—	57.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	25%	100.0	—	32.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	30%	100.0	—	18.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	35%	100.0	—	10.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	10%	56.2	—	48.1	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	20%	56.2	—	39.9	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	30%	56.2	—	36.6	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	40%	56.2	—	37.0	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	50%	56.2	—	37.5	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	60%	56.2	—	38.6	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	10%	56.2	—	49.3	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	20%	56.2	—	46.0	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	30%	56.2	—	47.0	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
WT	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	40%	56.2	—	50.4	°C	50 mM sodium phosphate buffer	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	5%	100.0	—	77.99	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	10%	100.0	—	71.84	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	15%	100.0	—	65.37	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	20%	100.0	—	49.84	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	25%	100.0	—	40.78	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	30%	100.0	—	28.8	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	40%	100.0	—	13.59	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	50%	100.0	—	3.88	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	60%	100.0	—	1.61	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	—	85.76	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	—	76.699	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	—	62.78	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	—	52.1	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	—	42.07	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	—	34.304	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100.0	—	29.77	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100.0	—	19.417	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	60%	100.0	—	4.53	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	5%	100.0	—	89.64	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	100.0	—	65.05	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	15%	100.0	—	54.69	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	20%	100.0	—	39.8	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	25%	100.0	—	26.54	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	30%	100.0	—	15.21	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	40%	100.0	—	8.09	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	50%	100.0	—	8.74	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	60%	100.0	—	1.29	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	—	154.3	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	—	213.6	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	—	288.3	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	—	225.2	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	—	217.4	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	—	197.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	—	119.4	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	—	73.8	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100.0	—	34.9	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	5%	100.0	—	133.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	100.0	—	145.6	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	15%	100.0	—	163.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	20%	100.0	—	193.1	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	25%	100.0	—	150.4	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	30%	100.0	—	99.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	40%	100.0	—	45.6	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	50%	100.0	—	17.4	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	60%	100.0	—	2.8	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	5%	100.0	—	103.9	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	10%	100.0	—	110.7	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	15%	100.0	—	131.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	20%	100.0	—	144.6	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	25%	100.0	—	164.0	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	30%	100.0	—	136.9	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	40%	100.0	—	58.2	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	50%	100.0	—	28.1	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	60%	100.0	—	10.6	%	50 mM sodium phosphate	7.2	25	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	10%	0.19	—	2.42	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	0.19	—	1.79	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	0.19	—	2.35	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	0.19	—	1.08	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	0.19	—	0.86	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	Km (in mM) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	10%	0.19	—	3.12	mM	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	10%	497.0	—	1101.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	497.0	—	630.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	497.0	—	1039.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	497.0	—	1441.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	497.0	—	1764.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat (in 1/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	10%	497.0	—	2209.0	1/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	10%	2616.0	—	455.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetonitrile	10%	2616.0	—	352.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethylformamide (DMF)	10%	2616.0	—	442.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	2616.0	—	1334.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	2616.0	—	2051.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Activity - Michaelis-Menten	kcat/Km (in mM/min) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Methanol	10%	2616.0	—	708.0	mM/min	50 mM sodium phosphate	7.2	25	p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in mM/min)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	10%	78.0	—	69.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	20%	78.0	—	66.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	30%	78.0	—	66.0	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	40%	78.0	—	66.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	50%	78.0	—	67.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetone	60%	78.0	—	69.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	10%	78.0	—	69.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	20%	78.0	—	61.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	30%	78.0	—	57.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	40%	78.0	—	56.9	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	50%	78.0	—	57.5	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Acetonitrile	60%	78.0	—	58.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	78.0	—	76.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	78.0	—	76.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	78.0	—	78.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	78.0	—	80.7	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethylformamide (DMF)	10%	78.0	—	71.4	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by DSC in the presence of organic solvent	Dimethylformamide (DMF)	20%	78.0	—	69.6	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	10%	78.0	—	70.5	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	20%	78.0	—	65.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	30%	78.0	—	64.6	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	40%	78.0	—	64.1	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	50%	78.0	—	65.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Isopropanol	60%	78.0	—	66.0	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	10%	78.0	—	72.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	20%	78.0	—	68.3	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	30%	78.0	—	65.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	40%	78.0	—	63.8	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	50%	78.0	—	64.0	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)
A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y	Stability - Tm	Tm measurement by circular dichroism in the presence of organic solvent	Methanol	60%	78.0	—	64.2	°C	50 mM sodium phosphate	7.2	—	—	—	—	—	Classical aqueous control (in °C)

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Mutations in this dataset (2) — Md. Zahid Kamal et al. (2013)

WT A15S/F17S/A20E/N89Y/G111D/L114P/A132D/M134E/M137P/I157M/S163P/N166Y

Visualization : Activity — Classical

Md. Zahid Kamal et al. (2013)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Activity — Michaelis-Menten

Md. Zahid Kamal et al. (2013)

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — T_m

Md. Zahid Kamal et al. (2013)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Haiyang Cui et al. (2020) — How to Engineer Organic Solvent Resistant Enzymes: Insights from Combined Molecular Dynamics and Directed Evolution Study

ChemCatChem · doi:10.1002/cctc.202000422 ↗ · Activity + Stability - Incubation

570 measurements ▼

Database ID

PDB: 1I6W ↗

Sequence Annotation

Explicit - Provided PDB Accesion Number (always 1 mutation compared to PDB sequence)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (570 measurements)

570 measurements — page 1 of 29

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
I12F	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12W	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12A	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12V	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12L	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12G	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	50	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12M	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12P	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	0	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12S	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12T	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12Y	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12C	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12N	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12Q	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12K	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12R	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	200	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12H	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	0	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12D	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	0	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
I12E	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	0	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
S77F	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	0	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)

I12F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water | Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)

I12W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

h156Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

h156Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

h156Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

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Mutations in this dataset (190) — Haiyang Cui et al. (2020)

▼

I12A I12C I12D I12E I12F I12G I12H I12K I12L I12M I12N I12P I12Q I12R I12S I12T I12V I12W I12Y S77A S77C S77D S77E S77F S77G S77H S77I S77K S77L S77M S77N S77P S77Q S77R S77T S77V S77W S77Y M78A M78C M78D M78E M78F M78G M78H M78I M78K M78L M78N M78P M78Q M78R M78S M78T M78V M78W M78Y A105C A105D A105E A105F A105G A105H A105I A105K A105L A105M A105N A105P A105Q A105R A105S A105T A105V A105W A105Y L108A L108C L108D L108E L108F L108G L108H L108I L108K L108M L108N L108P L108Q L108R L108S L108T L108V L108W L108Y D133A D133C D133E D133F D133G D133H D133I D133K D133L D133M D133N D133P D133Q D133R D133S D133T D133V D133W D133Y I135A I135C I135D I135E I135F I135G I135H I135K I135L I135M I135N I135P I135Q I135R I135S I135T I135V I135W I135Y V136A V136C V136D V136E V136F V136G V136H V136I V136K V136L V136M V136N V136P V136Q V136R V136S V136T V136W V136Y L140A L140C L140D L140E L140F L140G L140H L140I L140K L140M L140N L140P L140Q L140R L140S L140T L140V L140W L140Y H156A H156C H156D H156E H156F H156G H156I H156K H156L H156M H156N H156P H156Q H156R H156S H156T H156V H156W h156Y

Visualization : Activity + Stability — Incubation

Haiyang Cui et al. (2020)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Kim N. Ingenbosch et al. (2021) — Effect of Organic Solvents on the Structure and Activity of a Minimal Lipase

The Journal of Organic Chemistry · doi:10.1021/acs.joc.1c01136 ↗ · Activity - Classical Activity - Michaelis-Menten

39 measurements ▼

Database ID

UniProt: P37957 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli

Experimental Data (39 measurements)

39 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	142.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	70%	100.0	2.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	50%	100.0	4.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	40%	100.0	11.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	30%	100.0	25.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	25%	100.0	38.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	20%	100.0	52.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	15%	100.0	72.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	10%	100.0	78.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	5%	100.0	100.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	50%	100.0	1.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	40%	100.0	2.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	4.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	5.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	9.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	21.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	138.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	126.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	115.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	99.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone , Butyric Acid	—	—	Classical aqueous control (in %)

—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	142.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	70%	100.0	2.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	50%	100.0	4.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	40%	100.0	11.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	30%	100.0	25.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	25%	100.0	38.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	20%	100.0	52.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	15%	100.0	72.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	10%	100.0	78.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	5%	100.0	100.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	50%	100.0	1.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	40%	100.0	2.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	4.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	5.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	9.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	21.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100.0	138.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	126.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	115.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100.0	99.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	81.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	35%	100.0	60.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100.0	50.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	45%	100.0	33.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	26.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100.0	13.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	70%	100.0	2.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	5%	100.0	68.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	7.5%	100.0	54.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Classical	Activity measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	13.0	%	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in %)
—	Activity - Michaelis-Menten	Km (µM) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	69.0	62.0	µM	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM)
—	Activity - Michaelis-Menten	Km (µM) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	10%	69.0	125.0	µM	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM)
—	Activity - Michaelis-Menten	Km (µM) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	10%	69.0	74.0	µM	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM)
—	Activity - Michaelis-Menten	vmax (µM/s) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	0.028	0.061	µM/s	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM/s)
—	Activity - Michaelis-Menten	vmax (µM/s) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	10%	0.028	0.02	µM/s	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM/s)
—	Activity - Michaelis-Menten	vmax (µM/s) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	10%	0.028	0.025	µM/s	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in µM/s)
—	Activity - Michaelis-Menten	kcat/Km (1/(s*µM)) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	0.08	0.196	1/(s * µM)	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in 1/(s * µM))
—	Activity - Michaelis-Menten	kcat/Km (1/(s*µM)) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Acetonitrile	10%	0.08	0.034	1/(s * µM)	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in 1/(s * µM))
—	Activity - Michaelis-Menten	kcat/Km (1/(s*µM)) measured by fluorescence spectroscopy (4- methylumbelliferone fluoresence measurement, excitation at 327 nm, absorbance measurement at 449 nm) in the presence of organic solvent	Isopropanol	10%	0.08	0.067	1/(s * µM)	10 mM sodium phosphate buffer	7.7	Room temperature	0.1 mM 4-Methylumbelliferyl butyrate	4- methylumbelliferone, Butyric Acid	—	—	Classical aqueous control (in 1/(s * µM))

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Visualization : Activity — Classical

Kim N. Ingenbosch et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity — Michaelis-Menten

Kim N. Ingenbosch et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume.

Haiyang Cui et al. (2021) — CompassR Yields Highly Organic-Solvent-Tolerant Enzymes through Recombination of Compatible Substitutions

Chemistry–A European Journal · doi:10.1002/chem.202004471 ↗ · Activity + Stability - Incubation

235 measurements ▼

Database ID

UniProt: P37957 ↗

Sequence Annotation

Explicit - Provided PDB Accession Number (31 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (235 measurements)

235 measurements — page 1 of 12

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	10%	100.0	—	71.9	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	14%	100.0	—	52.1	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	18%	100.0	—	47.5	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	22%	100.0	—	42.0	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	26%	100.0	—	32.2	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	30%	100.0	—	21.9	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	40%	100.0	—	7.2	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	50%	100.0	—	5.2	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	60%	100.0	—	3.7	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	70%	100.0	—	1.7	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	1,4-Dioxane	80%	100.0	—	1.2	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	10%	100.0	—	83.5	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	20%	100.0	—	52.3	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	30%	100.0	—	30.1	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	40%	100.0	—	6.9	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	50%	100.0	—	2.9	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Isopropanol	60%	100.0	—	1.9	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	10%	100.0	—	79.1	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	20%	100.0	—	56.8	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617
WT	Activity + Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent	Acetone	30%	100.0	—	36.4	%	Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile	Incubation: 7.5, Assay: 7.5	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 900 rpm	Water-incubated control (in %), assay in the presence of organic solvent \| Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

10%

100.0

—

71.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Water-incubated control (in %), assay in the presence of organic solvent | Clear about assay in the presence of organic solvent and 100% being the assay in aqueous phase/some of the information about assay conditions obtained from 10.1002/bit.25617

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

14%

100.0

—

52.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

18%

100.0

—

47.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

22%

100.0

—

42.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

26%

100.0

—

32.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

30%

100.0

—

21.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

40%

100.0

—

7.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

50%

100.0

—

5.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

60%

100.0

—

3.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

70%

100.0

—

1.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

80%

100.0

—

1.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

10%

100.0

—

83.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

20%

100.0

—

52.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

30%

100.0

—

30.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

40%

100.0

—

6.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

50%

100.0

—

2.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

60%

100.0

—

1.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

10%

100.0

—

79.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

20%

100.0

—

56.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

30%

100.0

—

36.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

40%

100.0

—

13.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

50%

100.0

—

6.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

60%

100.0

—

6.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

10%

100.0

—

95.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

20%

100.0

—

54.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

30%

100.0

—

35.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

40%

100.0

—

21.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

50%

100.0

—

9.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

60%

100.0

—

6.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

10%

100.0

—

123.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

20%

100.0

—

85.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

30%

100.0

—

54.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

40%

100.0

—

36.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

50%

100.0

—

21.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

60%

100.0

—

13.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

—

81.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

—

64.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

—

59.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

100.0

—

59.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

100.0

—

54.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

100.0

—

55.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

—

87.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

—

74.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

—

63.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

100.0

—

56.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

100.0

—

48.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

100.0

—

43.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

10%

100.0

71.9

109.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

14%

100.0

52.1

119.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

18%

100.0

47.5

116.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

22%

100.0

42.0

112.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

26%

100.0

32.2

110.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

30%

100.0

21.9

97.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

40%

100.0

7.2

79.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

50%

100.0

5.2

57.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

60%

100.0

3.7

39.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

70%

100.0

1.7

23.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

80%

100.0

1.2

6.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

10%

100.0

71.9

95.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

14%

100.0

52.1

94.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

18%

100.0

47.5

92.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

22%

100.0

42.0

88.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

26%

100.0

32.2

87.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

30%

100.0

21.9

77.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

40%

100.0

7.2

60.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

50%

100.0

5.2

45.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

60%

100.0

3.7

16.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

70%

100.0

1.7

15.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

80%

100.0

1.2

9.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

10%

100.0

71.9

99.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

14%

100.0

52.1

101.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

18%

100.0

47.5

99.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

22%

100.0

42.0

94.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

26%

100.0

32.2

92.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

30%

100.0

21.9

77.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

40%

100.0

7.2

61.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

50%

100.0

5.2

34.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

60%

100.0

3.7

14.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

70%

100.0

1.7

22.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

80%

100.0

1.2

11.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

10%

100.0

71.9

107.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

14%

100.0

52.1

109.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

18%

100.0

47.5

111.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

22%

100.0

42.0

109.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

26%

100.0

32.2

113.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

30%

100.0

21.9

119.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

40%

100.0

7.2

98.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

50%

100.0

5.2

76.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

60%

100.0

3.7

31.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

70%

100.0

1.7

31.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

1,4-Dioxane

80%

100.0

1.2

24.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

10%

100.0

83.5

113.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

20%

100.0

52.3

74.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

30%

100.0

30.1

45.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

40%

100.0

6.9

18.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

50%

100.0

2.9

5.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

60%

100.0

1.9

2.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

10%

100.0

83.5

85.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

20%

100.0

52.3

97.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

30%

100.0

30.1

54.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

40%

100.0

6.9

24.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

50%

100.0

2.9

4.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

60%

100.0

1.9

1.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

10%

100.0

83.5

81.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

20%

100.0

52.3

88.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

30%

100.0

30.1

58.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

40%

100.0

6.9

28.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

50%

100.0

2.9

11.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

60%

100.0

1.9

4.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

10%

100.0

83.5

80.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

20%

100.0

52.3

77.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

30%

100.0

30.1

57.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

40%

100.0

6.9

26.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

50%

100.0

2.9

9.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Isopropanol

60%

100.0

1.9

4.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

10%

100.0

79.1

91.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

20%

100.0

56.8

66.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

30%

100.0

36.4

48.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

40%

100.0

13.9

16.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

50%

100.0

6.7

8.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

60%

100.0

6.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

10%

100.0

79.1

106.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

20%

100.0

56.8

65.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

30%

100.0

36.4

50.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

40%

100.0

13.9

28.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

50%

100.0

6.7

12.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

60%

100.0

6.2

12.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

10%

100.0

79.1

89.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

20%

100.0

56.8

57.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

30%

100.0

36.4

49.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

40%

100.0

13.9

30.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

50%

100.0

6.7

19.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

60%

100.0

6.2

18.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

10%

100.0

79.1

84.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

20%

100.0

56.8

81.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

30%

100.0

36.4

57.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

40%

100.0

13.9

43.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

50%

100.0

6.7

40.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Acetone

60%

100.0

6.2

36.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

10%

100.0

95.7

121.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

20%

100.0

54.0

77.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

30%

100.0

35.1

54.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

40%

100.0

21.4

33.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

50%

100.0

9.1

18.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

60%

100.0

6.8

10.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

10%

100.0

95.7

87.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

20%

100.0

54.0

73.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

30%

100.0

35.1

58.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

40%

100.0

21.4

33.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

50%

100.0

9.1

18.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

60%

100.0

6.8

10.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

10%

100.0

95.7

71.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

20%

100.0

54.0

64.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

30%

100.0

35.1

58.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

40%

100.0

21.4

38.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

50%

100.0

9.1

22.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

60%

100.0

6.8

11.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

10%

100.0

95.7

94.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

20%

100.0

54.0

76.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

30%

100.0

35.1

71.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

40%

100.0

21.4

43.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

50%

100.0

9.1

26.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Ethanol

60%

100.0

6.8

17.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

10%

100.0

123.0

96.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

20%

100.0

85.4

100.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

30%

100.0

54.0

68.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

40%

100.0

36.0

49.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

50%

100.0

21.1

27.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

60%

100.0

13.8

16.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

10%

100.0

123.0

90.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

20%

100.0

85.4

77.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

30%

100.0

54.0

57.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

40%

100.0

36.0

37.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

50%

100.0

21.1

23.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

60%

100.0

13.8

20.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

10%

100.0

123.0

97.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

20%

100.0

85.4

97.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

30%

100.0

54.0

74.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

40%

100.0

36.0

42.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

50%

100.0

21.1

35.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

60%

100.0

13.8

36.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

10%

100.0

123.0

108.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

20%

100.0

85.4

104.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

30%

100.0

54.0

88.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

40%

100.0

36.0

57.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

50%

100.0

21.1

35.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Methanol

60%

100.0

13.8

33.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

81.1

83.1

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

64.0

93.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

59.6

145.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

100.0

59.6

142.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

100.0

54.5

136.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

100.0

55.8

71.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

81.1

62.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

64.0

65.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

59.6

64.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

100.0

59.6

63.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

100.0

54.5

64.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

100.0

55.8

48.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

81.1

111.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

64.0

85.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

59.6

90.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

100.0

59.6

91.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

100.0

54.5

74.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

100.0

55.8

44.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

100.0

81.1

63.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

100.0

64.0

71.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

100.0

59.6

74.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

100.0

59.6

72.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

100.0

54.5

64.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

100.0

55.8

21.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

87.6

110.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

74.4

78.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

63.9

56.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

100.0

56.2

45.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

100.0

48.5

32.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

100.0

43.1

31.3

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

87.6

85.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

74.4

88.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

63.9

79.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

100.0

56.2

70.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

100.0

48.5

64.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

100.0

43.1

54.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

87.6

75.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

74.4

81.6

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

63.9

100.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

100.0

56.2

91.2

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

100.0

48.5

63.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K/M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

100.0

43.1

33.8

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

87.6

76.5

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

74.4

53.9

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

100.0

63.9

49.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

100.0

56.2

46.7

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

100.0

48.5

46.4

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

I12R/Y49R/E65H/N98R/K122E/L124K

Activity + Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

100.0

43.1

38.0

Incubation: 50 mM triEthanolamine buffer, Assay: 50 mM triEthanolamine buffer, 5% acetonitrile

Incubation: 7.5, Assay: 7.5

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 900 rpm

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Mutations in this dataset (5) — Haiyang Cui et al. (2021)

WT I12R/Y49R/E65H/N98R/K122E/L124K I12R/Y49R/E65H/N98R/K122E/L124K/M137K Y49R/E65H/N98R/K122E/L124K K122E/L124K

Visualization : Activity + Stability — Incubation

Haiyang Cui et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Kyoungseon Min et al. (2021) — Improving the organic solvent resistance of lipase a from Bacillus subtilis in water–ethanol solvent through rational surface engineering

Bioresource Technology · doi:10.1016/j.biortech.2021.125394 ↗ · Activity - Classical Stability - Incubation

39 measurements ▼

Database ID

PDB: 1I6W ↗

Sequence Annotation

Explicit - Provided PDB Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (39 measurements)

39 measurements — page 1 of 2

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	32.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	21.5	—	32.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
WT	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	—	40.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	40.2	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	3.8	32.8	2.4	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
S16G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	30.1	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38C	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	40.6	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	14.9	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	33.7	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38T	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	25.6	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
N48K	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	16.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	26.2	32.8	33.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108K	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	2.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	2.4	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108S	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	4.7	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108T	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	6.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	11.0	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
D40A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	8.0	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
V54A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	27.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
F58A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	6.9	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid , p-Nitrophenol	—	—	Classical aqueous control (in U/mg)

WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	32.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	21.5	—	32.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
WT	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	—	40.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	40.2	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	3.8	32.8	2.4	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
S16G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	30.1	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38C	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	40.6	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38E	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	14.9	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	33.7	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38T	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	25.6	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
N48K	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	16.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	26.2	32.8	33.8	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108K	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	2.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	2.4	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108S	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	4.7	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108T	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	6.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L108Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	11.0	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
D40A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	8.0	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
V54A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	27.5	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
F58A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	6.9	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
L114A	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	—	32.8	12.4	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
S16G	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	26.0	32.8	30.2	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
A38T	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	33.1	32.8	25.6	U/mg	50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	8	20°C	3 mM p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Classical aqueous control (in U/mg)
S16G	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	70.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
A38C	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	31.4	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
A38E	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	36.8	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
A38G	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	55.5	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
A38T	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	53.6	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
N48K	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	15.7	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108F	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	27.8	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108K	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	28.9	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108N	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	54.3	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108S	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	28.7	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108T	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	16.9	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L108Y	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	26.6	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
D40A	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	24.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
V54A	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	26.2	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
F58A	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	16.0	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
L114A	Stability - Incubation	Activity after incubation (6 hours at 20°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	40.0	15.5	%	Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer, 33.3 mM acetonitrile, 0.5% Triton X-100	Incubation: 8, Assay: 8	Incubation: 20°C, Assay: 20°C	p-Nitrophenyl caprylate	Caprylic Acid, p-Nitrophenol	—	—	Non-incubated control in the presence of organic solvent (in %) , assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase

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Mutations in this dataset (17) — Kyoungseon Min et al. (2021)

WT S16G A38C A38E A38G A38T D40A N48K V54A F58A L108F L108K L108N L108S L108T L108Y L114A

Visualization : Activity — Classical

Kyoungseon Min et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Stability — Incubation

Kyoungseon Min et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Haiyang Cui et al. (2021) — Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance

Angewandte Chemie International Edition · doi:10.1002/anie.202101642 ↗ · Activity - Michaelis-Menten Stability - Incubation

1353 measurements ▼

Database ID

PDB: 1I6W ↗

Sequence Annotation

Explicit - Provided PDB Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (1353 measurements)

1353 measurements — page 1 of 68

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	22%	0.51	—	0.57	mM	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in mM)
WT	Activity - Michaelis-Menten	kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	22%	162.0	—	84.0	1/min	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
WT	Activity - Michaelis-Menten	kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	22%	318.0	—	147.0	1/(mM * min)	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/(mM * min))
WT	Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	0.51	—	0.65	mM	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in mM)
WT	Activity - Michaelis-Menten	kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	162.0	—	74.0	1/min	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
WT	Activity - Michaelis-Menten	kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	318.0	—	114.0	1/(mM * min)	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/(mM * min))
WT	Activity - Michaelis-Menten	Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	2,2,2-Trifluoroethanol (TFE)	12%	0.51	—	0.78	mM	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in mM)
WT	Activity - Michaelis-Menten	kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	2,2,2-Trifluoroethanol (TFE)	12%	162.0	—	124.0	1/min	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/min)
WT	Activity - Michaelis-Menten	kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	2,2,2-Trifluoroethanol (TFE)	12%	318.0	—	159.0	1/(mM * min)	50 mM triethylamine buffer	7.4	25°C	p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in 1/(mM * min))
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	5%	—	100.0	72.1	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	10%	—	100.0	52.2	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	15%	—	100.0	47.6	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	20%	—	100.0	38.6	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	25%	—	100.0	31.7	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	30%	—	100.0	24.2	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	35%	—	100.0	15.7	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	40%	—	100.0	10.6	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	45%	—	100.0	9.8	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	1,4-Dioxane	50%	—	100.0	6.4	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water
WT	Stability - Incubation	Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	—	100.0	72.4	%	Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer	Incubation: 7.4, Assay: 7.4	Incubation: 25°C, Assay: 25°C	0.25 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	Incubation: 800 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

0.51

—

0.57

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

162.0

—

84.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

318.0

—

147.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

0.51

—

0.65

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

162.0

—

74.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

318.0

—

114.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

0.51

—

0.78

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

162.0

—

124.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

318.0

—

159.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

72.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

52.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

47.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

38.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

31.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

24.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

15.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

10.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

9.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

6.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

72.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

63.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

58.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

59.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

50.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

52.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

37.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

12.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

1.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

0.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

87.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

74.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

63.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

56.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

48.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

42.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

35.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

42.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

2.30

0.57

4.84

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

339.0

84.0

234.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

147.0

48.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

0.53

0.57

0.43

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

73.0

84.0

28.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

138.0

147.0

65.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

0.18

0.57

0.15

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

47.0

84.0

19.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

261.0

147.0

127.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

1.42

0.57

0.84

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

109.0

84.0

39.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

77.0

147.0

46.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

0.16

0.57

0.11

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

39.0

84.0

17.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

244.0

147.0

155.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

2.30

0.65

4.68

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

339.0

74.0

136.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

147.0

114.0

29.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

0.53

0.65

0.66

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

73.0

74.0

23.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

138.0

114.0

35.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

0.18

0.65

0.33

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

47.0

74.0

17.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

261.0

114.0

52.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

1.42

0.65

0.83

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

109.0

74.0

21.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

77.0

114.0

25.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

0.16

0.65

0.43

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

39.0

74.0

15.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

244.0

114.0

35.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

2.30

0.78

4.06

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

339.0

124.0

364.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

147.0

159.0

90.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

0.53

0.78

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

73.0

124.0

105.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

138.0

159.0

135.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

0.18

0.78

0.66

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

47.0

124.0

87.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

261.0

159.0

132.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

1.42

0.78

0.96

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

109.0

124.0

120.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

77.0

159.0

125.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

Km (mM), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

0.16

0.78

0.5

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in mM)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat (1/min), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

39.0

124.0

89.0

1/min

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/min)

D34K/D64K/K112E/D144K

Activity - Michaelis-Menten

kcat/Km (1/(mM*min)), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

244.0

159.0

178.0

1/(mM * min)

50 mM triethylamine buffer

7.4

25°C

p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Classical aqueous control (in 1/(mM * min))

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

230.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

290.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

390.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

140.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

180.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

430.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

300.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

420.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

160.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

270.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

660.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

530.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

760.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

180.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

130.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

240.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

170.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

300.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K//D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

410.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K//D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

490.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K//D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

560.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

240.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

160.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

220.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

210.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

210.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

340.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

380.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

410.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

540.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

126.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

108.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

118.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

106.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

89.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

65.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

62.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

43.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

49.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

45.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

109.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

96.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

92.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

91.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

66.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

67.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

51.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

38.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

47.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

45.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

112.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

101.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

86.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

82.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

60.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

54.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

32.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

45.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

30.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

22.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

110.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

99.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

96.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

89.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

77.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

63.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

59.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

47.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

45.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

43.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

—

100.0

91.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

10%

—

100.0

83.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

15%

—

100.0

110.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

20%

—

100.0

76.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

25%

—

100.0

60.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

30%

—

100.0

54.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

35%

—

100.0

44.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

40%

—

100.0

32.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

45%

—

100.0

30.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

50%

—

100.0

31.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

97.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

86.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

85.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

89.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

70.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

66.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

48.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

22.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

16.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

12.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

81.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

75.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

53.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

66.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

49.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

43.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

36.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

22.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

15.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

10.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

126.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

120.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

85.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

90.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

57.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

53.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

32.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

15.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

17.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

9.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

100.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

106.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

83.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

84.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

59.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

44.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

48.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

25.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

24.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

15.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

10%

—

100.0

96.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

20%

—

100.0

76.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

30%

—

100.0

71.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

40%

—

100.0

68.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

50%

—

100.0

55.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

55.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

70%

—

100.0

48.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

80%

—

100.0

22.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

90%

—

100.0

21.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

100%

—

100.0

15.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

115.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

79.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

69.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

67.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

47.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

54.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

38.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

43.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

116.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

97.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

10.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

16.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

5.8

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

146.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

145.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

129.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

117.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

92.7

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

98.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

101.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

83.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

128.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

129.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

109.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

115.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

106.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

91.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

86.6

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

80.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

137.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

121.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

—

100.0

118.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

10%

—

100.0

93.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

72.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

14%

—

100.0

82.9

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

16%

—

100.0

54.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K/D64K/K112E/D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

20%

—

100.0

29.1

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

1.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

1.49

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

1.94

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

0.97

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

1.07

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

1.49

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

1.32

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

1.24

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

0.99

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

1.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

0.99

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

1.4

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

1.5

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

1.09

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

0.89

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

1.0

1.43

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

1.0

1.3

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

1.0

1.2

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water

D34F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D34R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D64R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D144R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K35R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K61R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K112R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

h156Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

1,4-Dioxane

22%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

h156Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H10R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D40R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D43R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K44R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K70R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D91R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K95R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D118R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

D133R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

h156Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

H156R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

K170R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171F

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

50.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Y

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171W

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171A

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171V

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171L

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171I

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

200.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171G

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171C

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171M

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171P

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171S

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171T

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171N

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171Q

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

0.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171D

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171E

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171H

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171K

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

E171R

Stability - Incubation

Activity after incubation (2 hours at 25°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in the presence of organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100.0

Incubation: 50 mM triethylamine buffer, Assay: 50 mM triethylamine buffer

Incubation: 7.4, Assay: 7.4

Incubation: 25°C, Assay: 25°C

0.25 mM p-Nitrophenyl butyrate

Butyric Acid, p-Nitrophenol

—

Incubation: 800 rpm

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Mutations in this dataset (373) — Haiyang Cui et al. (2021)

▼

WT H10A H10C H10D H10E H10F H10G H10H H10I H10K H10L H10M H10N H10P H10Q H10R H10S H10T H10V H10W H10Y D34A D34C D34D D34E D34F D34G D34H D34I D34K D34K//D64K/D144K D34K/D144K D34K/D64K D34K/D64K/D144K D34K/D64K/K112E D34K/D64K/K112E/D144K D34K/K112E D34K/K112E/D144K D34L D34M D34N D34P D34Q D34R D34S D34T D34V D34W D34Y K35A K35C K35D K35E K35F K35G K35H K35I K35K K35L K35M K35N K35P K35Q K35R K35S K35T K35V K35W K35Y D40A D40C D40D D40E D40F D40G D40H D40I D40K D40L D40M D40N D40P D40Q D40R D40S D40T D40V D40W D40Y D43A D43C D43D D43E D43F D43G D43H D43I D43K D43L D43M D43N D43P D43Q D43R D43S D43T D43V D43W D43Y K44A K44C K44D K44E K44F K44G K44H K44I K44K K44L K44M K44N K44P K44Q K44R K44S K44T K44V K44W K44Y K61A K61C K61D K61E K61F K61G K61H K61I K61K K61L K61M K61N K61P K61Q K61R K61S K61T K61V K61W K61Y D64A D64C D64D D64E D64F D64G D64H D64I D64K D64K/D144K D64K/K112E D64K/K112E/D144K D64L D64M D64N D64P D64Q D64R D64S D64T D64V D64W D64Y K70A K70C K70D K70E K70F K70G K70H K70I K70K K70L K70M K70N K70P K70Q K70R K70S K70T K70V K70W K70Y D91A D91C D91D D91E D91F D91G D91H D91I D91K D91L D91M D91N D91P D91Q D91R D91S D91T D91V D91W D91Y K95A K95C K95D K95E K95F K95G K95H K95I K95K K95L K95M K95N K95P K95Q K95R K95S K95T K95V K95W K95Y K112A K112C K112D K112E K112E/D144K K112F K112G K112H K112I K112K K112L K112M K112N K112P K112Q K112R K112S K112T K112V K112W K112Y D118A D118C D118D D118E D118F D118G D118H D118I D118K D118L D118M D118N D118P D118Q D118R D118S D118T D118V D118W D118Y D133A D133C D133D D133E D133F D133G D133H D133I D133K D133L D133M D133N D133P D133Q D133R D133S D133T D133V D133W D133Y D144A D144C D144D D144E D144F D144G D144H D144I D144K D144L D144M D144N D144P D144Q D144R D144S D144T D144V D144W D144Y H156A H156C H156D H156E H156F H156G H156H H156I H156K H156L H156M H156N H156P H156Q H156R H156S H156T H156V H156W h156Y K170A K170C K170D K170E K170F K170G K170H K170I K170K K170L K170M K170N K170P K170Q K170R K170S K170T K170V K170W K170Y E171A E171C E171D E171E E171F E171G E171H E171I E171K E171L E171M E171N E171P E171Q E171R E171S E171T E171V E171W E171Y

Visualization : Activity — Michaelis-Menten

Haiyang Cui et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect — Michaelis-Menten

Mutation impact on kinetic parameters in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Stability — Incubation

Haiyang Cui et al. (2021)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Haiyang Cui et al. (2022) — Polar Substitutions on the Surface of a Lipase Substantially Improve Tolerance in Organic Solvents

ChemSusChem · doi:10.1002/cssc.202102551 ↗ · Activity + Stability - Incubation

4880 measurements ▼

Database ID

PDB: 1I6W ↗

Sequence Annotation

Explicit - Provided PDB Accesion Number (always 1 mutation compared to PDB sequence)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (4880 measurements)

4880 measurements — page 1 of 244

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
A1C	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1M	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1P	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1S	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1T	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1N	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
A1Q	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2C	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2M	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2P	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2S	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2T	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2N	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
E2Q	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3C	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3M	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3P	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3S	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3T	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)
H3N	Activity + Stability - Incubation	Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent	1,4-Dioxane	22%	—	100	100	%	Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer	Incubation: 7.4, Assay: 7.4	Incubation: Room temperature, Assay: Room temperature	0.5 mM p-Nitrophenyl butyrate	Butyric Acd , p-Nitrophenol	—	Incubation: 1000 rpm	Water-incubated control (in %), assay in the presence of organic solvent measuring the ratio between activity in the presence of organic solvent/activity in water \| Qualitative data (0 for no activity, 50 for activity reduction, 100 for retained activity, 200 for increased activity)

A1C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

1,4-Dioxane

22%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

2,2,2-Trifluoroethanol (TFE)

12%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A1Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E2Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H3Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N4Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P5Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V6Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V7Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M8Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V9Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H10Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G11Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I12Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G13Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G14Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A15Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S16R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F17Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N18Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F19N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A20Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G21Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I22Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K23Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S24R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y25Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L26Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V27Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S28R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q29R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G30Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W31Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S32R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R33Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D34Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K35Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L36Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y37Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A38Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V39Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D40Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F41Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

W42Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D43Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K44Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T45R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G46Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T47R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N48Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y49Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N50Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N51Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G52Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P53R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V54Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L55Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S56R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R57Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

F58Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V59Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q60R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K61Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V62Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L63Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D64Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E65Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T66R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G67Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A68Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K69Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K70Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V71Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D72Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I73Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V74Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A75Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H76Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S77R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M78R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G79Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G80Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A81Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N82Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T83Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L84Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y85Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y86Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I87Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K88Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N89Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L90Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D91Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G92Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G93Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N94Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K95Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V96Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A97Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N98Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V99Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V100Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T101Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L102Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G103Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G104Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A105Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N106Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R107Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L108Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T109R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T110R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G111Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K112Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A113Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L114Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P115R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G116Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T117R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D118Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

P119R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N120Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q121Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K122Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I123Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L124Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y125Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T126Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S127Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I128Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y129Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S130Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S131R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A132Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D133Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M134R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I135Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V136Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

M137R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N138Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y139Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L140Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S141Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R142Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L143Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

D144Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G145Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

A146Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

R147Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N148Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V149Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q150R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I151Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H152Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G153Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V154Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G155Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

H156Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I157Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G158Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L159Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L160Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Y161Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S162R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S163R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q164R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

V165Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N166Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

S167R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L168Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

I169Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

K170Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

E171N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G172Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

L173Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N174Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G175Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G176Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

G177Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

Q178R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N179Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

200

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180F

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Y

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180W

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180A

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180V

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180L

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180I

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180G

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180Q

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180D

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180E

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180H

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180K

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

T180R

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181C

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181M

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181P

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181S

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181T

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181N

Activity + Stability - Incubation

Activity after incubation (2 hours at room temperature) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) with organic solvent

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

N181Q

Activity + Stability - Incubation

—

Dimethyl Sulfoxide (DMSO)

60%

—

100

Incubation : 50 mM TEA buffer, Assay: 50 mM TEA buffer

Incubation: 7.4, Assay: 7.4

Incubation: Room temperature, Assay: Room temperature

0.5 mM p-Nitrophenyl butyrate

Butyric Acd, p-Nitrophenol

—

Incubation: 1000 rpm

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Mutations in this dataset (1631) — Haiyang Cui et al. (2022)

▼

A1C A1M A1N A1P A1Q A1S A1T E2C E2M E2N E2P E2Q E2S E2T H3C H3M H3N H3P H3Q H3S H3T N4C N4M N4N N4P N4Q N4S N4T P5C P5M P5N P5P P5Q P5S P5T V6C V6M V6N V6P V6Q V6S V6T V7C V7M V7N V7P V7Q V7S V7T M8C M8M M8N M8P M8Q M8S M8T V9C V9M V9N V9P V9Q V9S V9T H10C H10M H10N H10P H10Q H10S H10T G11C G11M G11N G11P G11Q G11S G11T I12C I12M I12N I12P I12Q I12S I12T G13C G13M G13N G13P G13Q G13S G13T G14C G14M G14N G14P G14Q G14S G14T A15C A15M A15N A15P A15Q A15S A15T S16A S16C S16D S16E S16F S16G S16H S16I S16K S16L S16M S16N S16P S16Q S16R S16S S16T S16V S16W S16Y F17C F17M F17N F17P F17Q F17S F17T N18C N18M N18N N18P N18Q N18S N18T F19C F19M F19N F19P F19Q F19S F19T A20C A20M A20N A20P A20Q A20S A20T G21C G21M G21N G21P G21Q G21S G21T I22C I22M I22N I22P I22Q I22S I22T K23C K23M K23N K23P K23Q K23S K23T S24A S24C S24D S24E S24F S24G S24H S24I S24K S24L S24M S24N S24P S24Q S24R S24S S24T S24V S24W S24Y Y25C Y25M Y25N Y25P Y25Q Y25S Y25T L26C L26M L26N L26P L26Q L26S L26T V27C V27M V27N V27P V27Q V27S V27T S28A S28C S28D S28E S28F S28G S28H S28I S28K S28L S28M S28N S28P S28Q S28R S28S S28T S28V S28W S28Y Q29A Q29C Q29D Q29E Q29F Q29G Q29H Q29I Q29K Q29L Q29M Q29N Q29P Q29Q Q29R Q29S Q29T Q29V Q29W Q29Y G30C G30M G30N G30P G30Q G30S G30T W31C W31M W31N W31P W31Q W31S W31T S32A S32C S32D S32E S32F S32G S32H S32I S32K S32L S32M S32N S32P S32Q S32R S32S S32T S32V S32W S32Y R33C R33M R33N R33P R33Q R33S R33T D34C D34M D34N D34P D34Q D34S D34T K35C K35M K35N K35P K35Q K35S K35T L36C L36M L36N L36P L36Q L36S L36T Y37C Y37M Y37N Y37P Y37Q Y37S Y37T A38C A38M A38N A38P A38Q A38S A38T V39C V39M V39N V39P V39Q V39S V39T D40C D40M D40N D40P D40Q D40S D40T F41C F41M F41N F41P F41Q F41S F41T W42C W42M W42N W42P W42Q W42S W42T D43C D43M D43N D43P D43Q D43S D43T K44C K44M K44N K44P K44Q K44S K44T T45A T45C T45D T45E T45F T45G T45H T45I T45K T45L T45M T45N T45P T45Q T45R T45S T45T T45V T45W T45Y G46C G46M G46N G46P G46Q G46S G46T T47A T47C T47D T47E T47F T47G T47H T47I T47K T47L T47M T47N T47P T47Q T47R T47S T47T T47V T47W T47Y N48C N48M N48N N48P N48Q N48S N48T Y49C Y49M Y49N Y49P Y49Q Y49S Y49T N50C N50M N50N N50P N50Q N50S N50T N51C N51M N51N N51P N51Q N51S N51T G52C G52M G52N G52P G52Q G52S G52T P53A P53C P53D P53E P53F P53G P53H P53I P53K P53L P53M P53N P53P P53Q P53R P53S P53T P53V P53W P53Y V54C V54M V54N V54P V54Q V54S V54T L55C L55M L55N L55P L55Q L55S L55T S56A S56C S56D S56E S56F S56G S56H S56I S56K S56L S56M S56N S56P S56Q S56R S56S S56T S56V S56W S56Y R57C R57M R57N R57P R57Q R57S R57T F58C F58M F58N F58P F58Q F58S F58T V59C V59M V59N V59P V59Q V59S V59T Q60A Q60C Q60D Q60E Q60F Q60G Q60H Q60I Q60K Q60L Q60M Q60N Q60P Q60Q Q60R Q60S Q60T Q60V Q60W Q60Y K61C K61M K61N K61P K61Q K61S K61T V62C V62M V62N V62P V62Q V62S V62T L63C L63M L63N L63P L63Q L63S L63T D64C D64M D64N D64P D64Q D64S D64T E65C E65M E65N E65P E65Q E65S E65T T66A T66C T66D T66E T66F T66G T66H T66I T66K T66L T66M T66N T66P T66Q T66R T66S T66T T66V T66W T66Y G67C G67M G67N G67P G67Q G67S G67T A68C A68M A68N A68P A68Q A68S A68T K69C K69M K69N K69P K69Q K69S K69T K70C K70M K70N K70P K70Q K70S K70T V71C V71M V71N V71P V71Q V71S V71T D72C D72M D72N D72P D72Q D72S D72T I73C I73M I73N I73P I73Q I73S I73T V74C V74M V74N V74P V74Q V74S V74T A75C A75M A75N A75P A75Q A75S A75T H76C H76M H76N H76P H76Q H76S H76T S77A S77C S77D S77E S77F S77G S77H S77I S77K S77L S77M S77N S77P S77Q S77R S77S S77T S77V S77W S77Y M78A M78C M78D M78E M78F M78G M78H M78I M78K M78L M78M M78N M78P M78Q M78R M78S M78T M78V M78W M78Y G79C G79M G79N G79P G79Q G79S G79T G80C G80M G80N G80P G80Q G80S G80T A81C A81M A81N A81P A81Q A81S A81T N82C N82M N82N N82P N82Q N82S N82T T83C T83M T83N T83P T83Q T83S T83T L84C L84M L84N L84P L84Q L84S L84T Y85C Y85M Y85N Y85P Y85Q Y85S Y85T Y86C Y86M Y86N Y86P Y86Q Y86S Y86T I87C I87M I87N I87P I87Q I87S I87T K88C K88M K88N K88P K88Q K88S K88T N89C N89M N89N N89P N89Q N89S N89T L90C L90M L90N L90P L90Q L90S L90T D91C D91M D91N D91P D91Q D91S D91T G92C G92M G92N G92P G92Q G92S G92T G93C G93M G93N G93P G93Q G93S G93T N94C N94M N94N N94P N94Q N94S N94T K95C K95M K95N K95P K95Q K95S K95T V96C V96M V96N V96P V96Q V96S V96T A97C A97M A97N A97P A97Q A97S A97T N98C N98M N98N N98P N98Q N98S N98T V99C V99M V99N V99P V99Q V99S V99T V100C V100M V100N V100P V100Q V100S V100T T101C T101M T101N T101P T101Q T101S T101T L102C L102M L102N L102P L102Q L102S L102T G103C G103M G103N G103P G103Q G103S G103T G104C G104M G104N G104P G104Q G104S G104T A105C A105M A105N A105P A105Q A105S A105T N106C N106M N106N N106P N106Q N106S N106T R107C R107M R107N R107P R107Q R107S R107T L108C L108M L108N L108P L108Q L108S L108T T109A T109C T109D T109E T109F T109G T109H T109I T109K T109L T109M T109N T109P T109Q T109R T109S T109T T109V T109W T109Y T110A T110C T110D T110E T110F T110G T110H T110I T110K T110L T110M T110N T110P T110Q T110R T110S T110T T110V T110W T110Y G111C G111M G111N G111P G111Q G111S G111T K112C K112M K112N K112P K112Q K112S K112T A113C A113M A113N A113P A113Q A113S A113T L114C L114M L114N L114P L114Q L114S L114T P115A P115C P115D P115E P115F P115G P115H P115I P115K P115L P115M P115N P115P P115Q P115R P115S P115T P115V P115W P115Y G116C G116M G116N G116P G116Q G116S G116T T117A T117C T117D T117E T117F T117G T117H T117I T117K T117L T117M T117N T117P T117Q T117R T117S T117T T117V T117W T117Y D118C D118M D118N D118P D118Q D118S D118T P119A P119C P119D P119E P119F P119G P119H P119I P119K P119L P119M P119N P119P P119Q P119R P119S P119T P119V P119W P119Y N120C N120M N120N N120P N120Q N120S N120T Q121C Q121M Q121N Q121P Q121Q Q121S Q121T K122C K122M K122N K122P K122Q K122S K122T I123C I123M I123N I123P I123Q I123S I123T L124C L124M L124N L124P L124Q L124S L124T Y125C Y125M Y125N Y125P Y125Q Y125S Y125T T126C T126M T126N T126P T126Q T126S T126T S127C S127M S127N S127P S127Q S127S S127T I128C I128M I128N I128P I128Q I128S I128T Y129C Y129M Y129N Y129P Y129Q Y129S Y129T S130C S130M S130N S130P S130Q S130S S130T S131A S131C S131D S131E S131F S131G S131H S131I S131K S131L S131M S131N S131P S131Q S131R S131S S131T S131V S131W S131Y A132C A132M A132N A132P A132Q A132S A132T D133C D133M D133N D133P D133Q D133S D133T M134A M134C M134D M134E M134F M134G M134H M134I M134K M134L M134M M134N M134P M134Q M134R M134S M134T M134V M134W M134Y I135C I135M I135N I135P I135Q I135S I135T V136C V136M V136N V136P V136Q V136S V136T M137A M137C M137D M137E M137F M137G M137H M137I M137K M137L M137M M137N M137P M137Q M137R M137S M137T M137V M137W M137Y N138C N138M N138N N138P N138Q N138S N138T Y139C Y139M Y139N Y139P Y139Q Y139S Y139T L140C L140M L140N L140P L140Q L140S L140T S141C S141M S141N S141P S141Q S141S S141T R142C R142M R142N R142P R142Q R142S R142T L143C L143M L143N L143P L143Q L143S L143T D144C D144M D144N D144P D144Q D144S D144T G145C G145M G145N G145P G145Q G145S G145T A146C A146M A146N A146P A146Q A146S A146T R147C R147M R147N R147P R147Q R147S R147T N148C N148M N148N N148P N148Q N148S N148T V149C V149M V149N V149P V149Q V149S V149T Q150A Q150C Q150D Q150E Q150F Q150G Q150H Q150I Q150K Q150L Q150M Q150N Q150P Q150Q Q150R Q150S Q150T Q150V Q150W Q150Y I151C I151M I151N I151P I151Q I151S I151T H152C H152M H152N H152P H152Q H152S H152T G153C G153M G153N G153P G153Q G153S G153T V154C V154M V154N V154P V154Q V154S V154T G155C G155M G155N G155P G155Q G155S G155T H156C H156M H156N H156P H156Q H156S H156T I157C I157M I157N I157P I157Q I157S I157T G158C G158M G158N G158P G158Q G158S G158T L159C L159M L159N L159P L159Q L159S L159T L160C L160M L160N L160P L160Q L160S L160T Y161C Y161M Y161N Y161P Y161Q Y161S Y161T S162A S162C S162D S162E S162F S162G S162H S162I S162K S162L S162M S162N S162P S162Q S162R S162S S162T S162V S162W S162Y S163A S163C S163D S163E S163F S163G S163H S163I S163K S163L S163M S163N S163P S163Q S163R S163S S163T S163V S163W S163Y Q164A Q164C Q164D Q164E Q164F Q164G Q164H Q164I Q164K Q164L Q164M Q164N Q164P Q164Q Q164R Q164S Q164T Q164V Q164W Q164Y V165C V165M V165N V165P V165Q V165S V165T N166C N166M N166N N166P N166Q N166S N166T S167A S167C S167D S167E S167F S167G S167H S167I S167K S167L S167M S167N S167P S167Q S167R S167S S167T S167V S167W S167Y L168C L168M L168N L168P L168Q L168S L168T I169C I169M I169N I169P I169Q I169S I169T K170C K170M K170N K170P K170Q K170S K170T E171C E171M E171N E171P E171Q E171S E171T G172C G172M G172N G172P G172Q G172S G172T L173C L173M L173N L173P L173Q L173S L173T N174C N174M N174N N174P N174Q N174S N174T G175C G175M G175N G175P G175Q G175S G175T G176C G176M G176N G176P G176Q G176S G176T G177C G177M G177N G177P G177Q G177S G177T Q178A Q178C Q178D Q178E Q178F Q178G Q178H Q178I Q178K Q178L Q178M Q178N Q178P Q178Q Q178R Q178S Q178T Q178V Q178W Q178Y N179C N179M N179N N179P N179Q N179S N179T T180A T180C T180D T180E T180F T180G T180H T180I T180K T180L T180M T180N T180P T180Q T180R T180S T180T T180V T180W T180Y N181C N181M N181N N181P N181Q N181S N181T

Visualization : Activity + Stability — Incubation

Haiyang Cui et al. (2022)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Lipase A (gene lipA) from Bacillus subtilis

Enzyme Description

Sequence

Experimental Data (105 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Stability — Half-life

Mutation Effect

Experimental Data (124 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Activity — Michaelis-Menten

Visualization : Stability — Tm

Mutation Effect

Experimental Data (570 measurements)

Visualization : Activity + Stability — Incubation

Mutation Effect

Experimental Data (39 measurements)

Visualization : Activity — Classical

Visualization : Activity — Michaelis-Menten

Experimental Data (235 measurements)

Visualization : Activity + Stability — Incubation

Mutation Effect

Experimental Data (39 measurements)

Visualization : Activity — Classical

Mutation Effect

Visualization : Stability — Incubation

Mutation Effect

Experimental Data (1353 measurements)

Visualization : Activity — Michaelis-Menten

Mutation Effect — Michaelis-Menten

Visualization : Stability — Incubation

Mutation Effect

Experimental Data (4880 measurements)

Visualization : Activity + Stability — Incubation

Mutation Effect

Structure

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Visualization : Stability — T_m