Lipase (gene lip1) from Fervidobacterium changbaicum CBS-1
Enzyme Description
Extremophile
Yes
"hyperthermophilic" organism
EC Number
Sequence
MENNTFVSFLKVLLLGTIVFYISTHLSTSRIVEYVSQDAKKLTIEGIQVAYREYGKGNFETIVFLHGFAGSSYDWKVLIDVLSENYHCIAFDIPPFGLSEKKNDFDYSDESIVRLLIKSLDSLGIEQFTLVGHSMGGYLSLAIASIIPKRVERLILFDAAYDVNSEDLQNPGPPFKLKDEHLLKFYQIFLDVGLKTYPLFKFVYRNSLAEGEILNAEHFDYLFSQNYFLPAEILIKFTKDKAAQKPLKIDLEGITAKTLIIYGEKDQITPPSIGEYLSKSIKNSKFMLIPNEGHMPLSNRLVIELVRKFLIDILE
Jingang Cai et al. (2010)
β Fervidobacterium changbaicum Lip1: identification, cloning, and characterization of the thermophilic lipase as a new member of bacterial lipase family V
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-010-2971-y β
Β· Activity - Classical Activity + Stability - Incubation
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Database ID
UniProt: A1YV97 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (30 measurements)
30 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 20% | 100.0 | 67.7 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 5% | 100.0 | 89.7 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 120.2 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 99.7 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 101.8 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 92.4 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 20% | 100.0 | 106.7 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 5% | 100.0 | 89.7 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Propanol | 20% | 100.0 | 123.4 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Propanol | 5% | 100.0 | 90.6 | % | 50 mM PBS | 7.8 | 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %), activity measured in the presence of organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 20% | 100.0 | 84.6 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 5% | 100.0 | 71.4 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 37.8 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 5% | 100.0 | 31.3 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 20% | 100.0 | 86.6 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Acetone | 5% | 100.0 | 69.1 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Propanol | 20% | 100.0 | 124.5 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | 1-Propanol | 5% | 100.0 | 78.8 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 20% | 100.0 | 46.7 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
| Activity + Stability - Incubation | Activity after incubation (24 hours at 37Β°C) measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 5% | 100.0 | 78.4 | % | Incubation: 50 mM PBS, Assay: 50 mM PBS | Incubation: 7.8, Assay: 7.8 | Incubation: 37Β°C, Assay: 70Β°C | ? mM p-Nitrophenyl caprylate | Caprylic Acid , p-Nitrophenol | β | Incubation: 150 rpm | Water-incubated control (in %), activity measured in the presence of organic solvent | High uncertainty about whether residual activity assay was with or without organic solvent |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Activity + Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.