Glucose 1-dehydrogenase from Lysinibacillus sphaericus G10

Enzyme Description

Species
Lysinibacillus sphaericus G10 β†—
Extremophile
No but "organic-solvent tolerant and alkali-resistant" enzyme
EC Number
1.1.1.47 β†—

Sequence

Length: 261 amino acids
MYSDLEGKVIVITGASTGLGKAMALRFGEEKAKVIVNFRSDENEANAVVEGVKKAGGDAIAVKGDVTVEEDVINLVQTAVNKFGTLDVMINNAGIENPVASHEMPLSDWNRVINTNLTGAFLGSREAIKYFVENDIKGSVINMSSVHEMIPWPLFVHYAASKGGIKLMTQTLALEYAPKGIRINNIGPGAINTPINAEKFADPAKRADVESMVPMGYIGKPEEIAAVAAWLASSQASYVTGITLFADGGMTLYPDFQAGRG
Hai-Tao Ding et al. (2011) β€” Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10
Bioresource Technology  Β· doi:10.1016/j.biortech.2010.08.018 β†—  Β· Stability - Incubation
80 measurements ▼
Database ID
UniProt: C5IFU0 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (80 measurements)

80 measurements β€” page 4 of 4
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Dimethyl Sulfoxide (DMSO) 40% 100.0 104.0 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry n-Hexane 40% 100.0 6.2 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Hexanol 40% 100.0 88.0 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Pentanol 40% 100.0 70.0 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Butanol 40% 100.0 1.5 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Isopropanol 40% 100.0 10.2 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Propanol 40% 100.0 0.0 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Ethanol 40% 100.0 0.6 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Methanol 40% 100.0 34.9 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Acetone 40% 100.0 1.5 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) Dimethyl Sulfoxide (DMSO) 10% 100.0 99.4 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry n-Hexane 30% 100.0 100.6 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Hexanol 30% 100.0 83.8 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Pentanol 30% 100.0 67.4 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Butanol 30% 100.0 1.4 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Isopropanol 30% 100.0 96.2 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry 1-Propanol 30% 100.0 0.0 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Ethanol 30% 100.0 101.2 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Methanol 30% 100.0 100.8 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
Stability - Incubation Activity after incubation (1 hour at 30Β°C) measured by absorbance spectrophotometry Acetone 30% 100.0 100.1 % Incubation: ?, Assay: 100 mM sodium phosphate buffer Incubation: ?, Assay: 8 Incubation: 25Β°C, Assay: 25Β°C 200 mM D-Glucose D-glucono-1,5-lactone (which hydrolyzes to D-gluconic acid) 1 mM NAD+ β€” Water-incubated control (in %), activity measured in aquous phase | Uncertainty about whether activity was really measured in aqueous phase and control
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Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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