Protease from Serratia marcescens MH6
Enzyme Description
Sequence
MSICLIDINQVMSGIEPMQSTKKAIEITESSLAAATTGYDAVDDLLHYHERGNGIQINGKDSFSNEQAGLFITRENQTWNGYKVFGQPVKLTFSFPDYKFSSTNVAGDTGLSKFSAEQQQQAKLSLQSWADVANITFTEVAAGQKANITFGNYSQDRPGHYDYGTQAYAFLPNTIWQGQDLGGQTWYNVNQSNVKHPATEDYGRQTFTHEIGHALGLSHPGDYNAGEGNPTYNDVTYAEDTRQFSLMSYWSETNTGGDNGGHYAAAPLLDDIAAIQHLYGANLSTRTGDTVYGFNSNTGRDFLSTTSNSQKVIFAAWDAGGNDTFDFSGYTANQRINLNEKSFSDVGGLKGNVSIAAGVTIENAIGGSGNDVIVGNAANNVLKGGAGNDVLFGGGGADELWGGAGKDIFVFSAASDSAPGASDWIRDFQKGIDKIDLSFFNKEANSSDFIHFVDHFSGTAGEALLSYNASSNVTDLSVNIGGHQAPDFLVKIVGQVDVATDFIV
Mao-Hua Wan et al. (2010)
β Screening, Characterization, and Cloning of a Solvent-Tolerant Protease from Serratia marcescens MH6
Journal of Microbiology and Biotechnology
Β· doi:10.4014/jmb.0910.10038 β
Β· Stability - Incubation
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Database ID
UniProt: A0A346M6V2 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Serratia marcescens MH6
Experimental Data (34 measurements)
34 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100 | 24 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Tetradecane | 50% | 100 | 120 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Dodecane | 50% | 100 | 103 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Decane | 50% | 100 | 100 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Octane | 50% | 100 | 122 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Heptane | 50% | 100 | 107 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Cyclohexane | 50% | 100 | 94 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Ethyl Acetate | 50% | 100 | 137 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 50 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Hexadecane | 50% | 100 | 116 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Isopropanol | 50% | 100 | 10 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Butanol | 50% | 100 | 53 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Isoamyl Alcohol | 50% | 100 | 100 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Heptanol | 50% | 100 | 112 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Octanol | 50% | 100 | 150 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Decanol | 50% | 100 | 153 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | 1-Dodecanol | 50% | 100 | 133 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | Dimethylformamide (DMF) | 25% | 100 | 52 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Tetradecane | 25% | 100 | 112 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
| Stability - Incubation | Activity after incubation (48 hours at 30Β°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 280 nm) in aqueous phase | n-Dodecane | 25% | 100 | 108 | % | Incubation: 50 mM Tris-HCl, Assay: 50 mM Tris-HCl | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 40Β°C | 2% (w/v) Casein | free amino acids , Peptides | β | Incubation: 180 rpm | Non-incubated control (in %), activity measured in aqueous phase | Uncertainty in particular about control type |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.