Lipase from Acinetobacter baylyi

Enzyme Description

Species
Acinetobacter baylyi β†—
Extremophile
No but "thermophilic" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 323 amino acids
MKVKFLLTGILIVLAQPVLASSAPIQNAKTSFVISSYAQTKYPIVLAHGLFGFNKLGTEAFGLDYWYQIPQDLAKNGANVWATRQSAANTSEVRGEQLLAEVQDILAITGAQKVNLIGHSHGSQSVRYVAGVLPDRVASVSTVGGPAKGAPLADLIYKSLAGTVLEARNTGQCSHQLHTIGQLDDPRQYPMDSLGAAYSLSTEGANKFNATFSAGVPMPTTACGQGAAKVNGIQYYSWSGASVLTNPVDPTDYLLGLTSVFSGQNNDGLVPACSSHIGTVIRDNYVLNHLDEVNQVLGLRSVFAQDPVSIFRQHANRLKLQGL
Sasithorn Uttatree et al. (2010) β€” Isolation and Characterization of a Novel Thermophilic-Organic Solvent Stable Lipase From Acinetobacter baylyi
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-010-8928-x β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniProt: H9U039 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Acinetobacter baylyi

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 92 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Ethanol 50% 100 60 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Acetonitrile 50% 100 66 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Isopropanol 50% 100 59 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Methanol 50% 100 64 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase 1-Butanol 50% 100 60 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Isoamyl Alcohol 50% 100 108 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase Benzene 50% 100 100 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Hexane 50% 100 72 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Heptane 50% 100 92 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Decane 50% 100 75 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 37Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in aqueous phase n-Hexadecane 50% 100 81 % Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer Incubation: 8, Assay: 8 37Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” Incubation: 150 rpm Non-incubated control (in %), activity measured in aqueous phase

Visualization : Stability β€” Incubation

Sasithorn Uttatree et al. (2010)

One bar per measurement. Colour = solvent, shade = solvent volume.

Sasithorn Uttatree et al. (2011) β€” Nutritional Requirements and Physical Factors Affecting the Production of Organic Solvent-Stable Lipase by Acinetobacter baylyi
Chiang Mai University Journal of Natural Sciences  Β· doi:https://www.thaiscience.info/journals/Article/CMUJ/10887586.pdf β†—  Β· Stability - Incubation
72 measurements ▼

Structure

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