Lipase from Pseudomonas aeruginosa LST-03

Enzyme Description

Species

Extremophile

EC Number

3.1.1.3 ↗

Sequence

Length: 285 amino acids

STYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL

Takuya Kawata et al. (2009) — Enhancement of the organic solvent-stability of the LST-03 lipase by directed evolution

Biotechnology Progress · doi:10.1002/btpr.264 ↗ · Stability - Incubation Stability - Half-life

40 measurements ▼

Database ID

UniProt: A8QYB2 ↗

Sequence Annotation

Explicit - Provided (26 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli JM109

Experimental Data (40 measurements)

40 measurements — page 2 of 2

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
S155L	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	n-Octane	25%	—	6.6	31.6	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
L145H	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	n-Octane	25%	—	6.6	6.8	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
S155L	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	Cyclohexane	25%	—	6.0	15.8	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
S155L/G157R/G177V/S194R/S202W/D209N	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)	Cyclohexane	25%	—	6.0	41.1	days	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Classical aqueous control (in days)
S155L/G157R/G177V/S194R/S202W/D209N	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Decane	25%	1.0	0.22	0.47	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Decane	25%	1.0	0.22	0.28	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
L145H	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Decane	25%	1.0	—	0.22	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Octane	25%	1.0	0.22	0.82	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S164K/Y188F/S211R	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Octane	25%	1.0	0.22	0.65	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
R88	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Octane	25%	1.0	0.22	0.43	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S164K/Y188F/S211R	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Decane	25%	1.0	0.22	0.8	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
L145H	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	n-Octane	25%	1.0	—	0.22	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S164K/Y188F/S211R	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Cyclohexane	25%	1.0	0.29	0.83	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L/G157R/G177V/S194R/S202W/D209N	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Cyclohexane	25%	1.0	0.29	0.82	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Cyclohexane	25%	1.0	0.29	0.48	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
L145H	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Cyclohexane	25%	1.0	0.29	0.32	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
L145H	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	1.0	0.66	0.4	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S164K/Y188F/S211R	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	1.0	0.66	0.43	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	1.0	0.66	0.59	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent
S155L/G157R/G177V/S194R/S202W/D209N	Stability - Incubation	Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	1.0	—	0.66	relative units	Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB	Incubation: 8.5, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	butyrate , Free thiols	—	Incubation: 160 rpm	Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent \| Uncertainty about whether activity and control were really measured in the presence of organic solvent

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

—

6.9

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

—

6.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Dodecane

25%

—

8.9

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

—

6.6

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Incubation

Activity after incubation (14 days at 30°C), measured by absorance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm) in the presence of organic solvent

n-Octane

25%

1.0

0.22

0.24

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Non-incubated control (in relative units) in the presence of organic solvent, assay in the presence of organic solvent | Uncertainty about whether activity and control were really measured in the presence of organic solvent

Stability - Incubation

Cyclohexane

25%

1.0

—

0.29

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

1.0

0.66

0.27

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Stability - Incubation

n-Decane

25%

1.0

0.22

0.3

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S164K/Y188F/S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

—

6.0

54.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

—

6.9

11.2

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

—

6.9

17.4

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

—

6.9

19.7

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K/Y188F/S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

—

6.9

12.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K/Y188F/S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Dodecane

25%

—

8.9

97.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Dodecane

25%

—

8.9

12.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Dodecane

25%

—

8.9

9.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Dodecane

25%

—

8.9

6.9

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

—

6.0

7.0

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K/Y188F/S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

—

6.6

21.3

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

—

6.6

15.9

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

—

6.6

31.6

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

n-Octane

25%

—

6.6

6.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

—

6.0

15.8

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (colorimetric assay, free thiols and DTNB reaction product absorbance measurement, 412 nm)

Cyclohexane

25%

—

6.0

41.1

days

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Incubation

n-Decane

25%

1.0

0.22

0.47

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L

Stability - Incubation

n-Decane

25%

1.0

0.22

0.28

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

L145H

Stability - Incubation

n-Decane

25%

1.0

—

0.22

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L

Stability - Incubation

n-Octane

25%

1.0

0.22

0.82

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S164K/Y188F/S211R

Stability - Incubation

n-Octane

25%

1.0

0.22

0.65

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

R88

Stability - Incubation

n-Octane

25%

1.0

0.22

0.43

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S164K/Y188F/S211R

Stability - Incubation

n-Decane

25%

1.0

0.22

0.8

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

L145H

Stability - Incubation

n-Octane

25%

1.0

—

0.22

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S164K/Y188F/S211R

Stability - Incubation

Cyclohexane

25%

1.0

0.29

0.83

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Incubation

Cyclohexane

25%

1.0

0.29

0.82

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L

Stability - Incubation

Cyclohexane

25%

1.0

0.29

0.48

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

L145H

Stability - Incubation

Cyclohexane

25%

1.0

0.29

0.32

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

L145H

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

1.0

0.66

0.4

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S164K/Y188F/S211R

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

1.0

0.66

0.43

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

1.0

0.66

0.59

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

S155L/G157R/G177V/S194R/S202W/D209N

Stability - Incubation

Dimethyl Sulfoxide (DMSO)

25%

1.0

—

0.66

relative units

Incubation: 10 mM Tris-HCl, Assay: 100 mM Tris-HCl , 0.3 mM DTNB

Incubation: 8.5, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

butyrate, Free thiols

—

Incubation: 160 rpm

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Mutations in this dataset (6) — Takuya Kawata et al. (2009)

WT R88 L145H S155L S155L/G157R/G177V/S194R/S202W/D209N S164K/Y188F/S211R

Visualization : Stability — Incubation

Takuya Kawata et al. (2009)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Visualization : Stability — Half-life

Takuya Kawata et al. (2009)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Takuya Kawata et al. (2010) — Amino acid residues involved in organic solvent-stability of the LST-03 lipase

Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.08.080 ↗ · Stability - Half-life

130 measurements ▼

Database ID

UniProt: A8QYB2 ↗

Sequence Annotation

Inferred - from protein name (26 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli JM109

Experimental Data (130 measurements)

130 measurements — page 1 of 7

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Decane	25%	5.3	—	8.9	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Octane	25%	5.3	—	6.6	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Heptane	25%	5.3	—	9.5	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Hexane	25%	5.3	—	13.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Cyclohexane	25%	5.3	—	8.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Toluene	25%	5.3	—	26.6	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Ethylene Glycol	25%	5.3	—	78.8	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Triethylene Glycol	25%	5.3	—	<1.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Ethanol	25%	5.3	—	<1.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Methanol	25%	5.3	—	<1.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Dimethyl Sulfoxide (DMSO)	25%	5.3	—	6.9	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Dimethylformamide (DMF)	25%	5.3	—	<1.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
WT	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Glycerol	25%	5.3	—	>100.0	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Decane	25%	7.2	8.9	10.5	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Octane	25%	7.2	6.6	18.7	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Heptane	25%	7.2	9.5	17.2	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	n-Hexane	25%	7.2	13.0	10.6	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Cyclohexane	25%	7.2	8.0	10.6	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Toluene	25%	7.2	26.6	16.7	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)
S211R	Stability - Half-life	Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)	Ethylene Glycol	25%	7.2	78.8	8.2	days	Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB	Incubation: ?, Assay: 8	Incubation: 30°C, Assay: 30°C	2 mM 2,3-Dimercapto-1-propanol tributyrate	2,3-dimercaptopropan-1-ol , 3-butyrate	—	Incubation: 160 rpm	Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

5.3

—

8.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

5.3

—

6.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

5.3

—

9.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

5.3

—

13.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

5.3

—

8.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

5.3

—

26.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

5.3

—

78.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

5.3

—

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

5.3

—

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

5.3

—

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

5.3

—

6.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

5.3

—

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

5.3

—

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

7.2

8.9

10.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

7.2

6.6

18.7

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

7.2

9.5

17.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

7.2

13.0

10.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

7.2

8.0

10.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

7.2

26.6

16.7

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

7.2

78.8

8.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

7.2

<1.0

10.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

7.2

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

7.2

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

7.2

6.9

9.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

7.2

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S211R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

7.2

>100.0

35.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

19.7

8.9

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

19.7

6.6

37.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

19.7

9.5

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

19.7

13.0

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

19.7

8.0

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

19.7

26.6

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

19.7

78.8

15.3

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

19.7

6.9

18.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

D209N

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

19.7

>100.0

39.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

3.6

8.9

10.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

3.6

6.6

8.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

3.6

9.5

8.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

3.6

13.0

12.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

3.6

8.0

12.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

3.6

26.6

12.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

3.6

78.8

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

3.6

<1.0

6.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

3.6

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

3.6

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

3.6

6.9

7.1

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

3.6

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S202W

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

3.6

>100.0

18.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

9.9

8.9

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

9.9

6.6

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

9.9

9.5

33.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

9.9

13.0

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

9.9

8.0

44.3

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

9.9

26.6

17.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

9.9

78.8

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

9.9

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

9.9

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

9.9

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

9.9

6.9

4.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

9.9

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S194R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

9.9

>100.0

38.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

4.3

8.9

4.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

4.3

6.6

3.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

4.3

9.5

3.7

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

4.3

13.0

3.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

4.3

8.0

3.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

4.3

26.6

7.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

4.3

78.8

8.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

4.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

4.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

4.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

4.3

6.9

4.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

4.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

Y188F

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

4.3

>100.0

38.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

4.0

8.9

4.7

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

4.0

6.6

10.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

4.0

9.5

7.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

4.0

13.0

4.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

4.0

8.0

9.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

4.0

26.6

17.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

4.0

78.8

15.3

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

4.0

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

4.0

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

4.0

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

4.0

6.9

14.3

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

4.0

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S164K

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

4.0

>100.0

39.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

12.4

8.9

16.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

12.4

6.6

25.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

12.4

9.5

10.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

12.4

13.0

28.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

12.4

8.0

21.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

12.4

26.6

13.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

12.4

78.8

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

12.4

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

12.4

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

12.4

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

12.4

6.9

10.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

12.4

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

G157R

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

12.4

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

19.7

8.9

9.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

19.7

6.6

31.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

19.7

9.5

>100.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

19.7

13.0

28.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

19.7

8.0

15.8

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

19.7

26.6

27.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

19.7

78.8

38.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

19.7

6.9

17.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

19.7

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

S155L

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

19.7

>100.0

49.5

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Decane

25%

6.3

8.9

6.9

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Octane

25%

6.3

6.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Heptane

25%

6.3

9.5

9.3

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

n-Hexane

25%

6.3

13.0

7.7

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Cyclohexane

25%

6.3

8.0

7.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Toluene

25%

6.3

26.6

8.4

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethylene Glycol

25%

6.3

78.8

23.6

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Triethylene Glycol

25%

6.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Ethanol

25%

6.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Methanol

25%

6.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethyl Sulfoxide (DMSO)

25%

6.3

6.9

11.2

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Dimethylformamide (DMF)

25%

6.3

<1.0

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

L145H

Stability - Half-life

Half-life (in days), with remaining activity measured by absorbance spectrophotometry (5-thio-2-nitrobenzoate absorbance measurement, 412 nm)

Glycerol

25%

6.3

>100.0

18.1

days

Incubation: Crude enzyme solution, Assay: 100 mM Tris-HCl, 0.3 mM DTNB

Incubation: ?, Assay: 8

Incubation: 30°C, Assay: 30°C

2 mM 2,3-Dimercapto-1-propanol tributyrate

2,3-dimercaptopropan-1-ol, 3-butyrate

—

Incubation: 160 rpm

Classical aqueous control (in days)

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Mutations in this dataset (10) — Takuya Kawata et al. (2010)

WT L145H S155L G157R S164K Y188F S194R S202W D209N S211R

Visualization : Stability — Half-life

Takuya Kawata et al. (2010)

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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Lipase from Pseudomonas aeruginosa LST-03

Enzyme Description

Sequence

Experimental Data (40 measurements)

Visualization : Stability — Incubation

Mutation Effect

Visualization : Stability — Half-life

Mutation Effect

Experimental Data (130 measurements)

Visualization : Stability — Half-life

Mutation Effect

Structure

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