Polyphenol oxidase from Agaricus bisporus

Enzyme Description

Species

Extremophile

EC Number

1.14.18.1 ↗

Sequence

Length: 568 amino acids

MSHLLVSPLGGGVQPRLEINNFVKNDRQFSLYVQALDRMYATPQNETASYFQVAGVHGYPLIPFDDAVGPTEFSPFDQWTGYCTHGSTLFPTWHRPYVLILEQILSGHAQQIADTYTVNKSEWKKAATEFRHPYWDWASNSVPPPEVISLPKVTITTPNGQKTSVANPLMRYTFNSVNDGGFYGPYNQWDTTLRQPDSTGVNAKDNVNRLKSVLKNAQASLTRATYDMFNRVTTWPHFSSHTPASGGSTSNSIEAIHDNIHVLVGGNGHMSDPSVAPFDPIFFLHHANVDRLIALWSAIRYDVWTSPGDAQFGTYTLRYKQSVDESTDLAPWWKTQNEYWKSNELRSTESLGYTYPEFVGLDMYNKDAVNKTISRKVAQLYGPQRGGQRSLVEDLSNSHARRSQRPAKRSRLGQLLKGLFSDWSAQIKFNRHEVGQSFSVCLFLGNVPEDPREWLVSPNLVGARHAFVRSVKTDHVAEEIGFIPINQWIAEHTGLPSFAVDLVKPLLAQGLQWRVLLADGTPAELDSLEVTILEVPSELTDDEPNPRSRPPRYHKDITHGKRGGCREA

Vered Shuster et al. (2009) — Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium ;

Microbial Physiology · doi:10.1159/000233506 ↗ · Activity - Classical Stability - Incubation

46 measurements ▼

Database ID

UniProt: Q00024 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Commercially purchased

Experimental Data (46 measurements)

46 measurements — page 3 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	60%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	61	26	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	10%	61	37	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)

Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	100	35	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	6	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	21	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	66	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	84	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	85	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	50%	100	19	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	40%	100	41	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	30%	100	48	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	20%	100	57	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Acetone	10%	100	78	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	50%	100	29	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	40%	100	44	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	20%	100	87	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	30%	100	52	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	10%	100	65	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	100	20	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	100	12	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	100	10	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	10%	100	48	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	20%	100	38	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	30%	100	35	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	40%	100	28	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Methanol	50%	100	13	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorabce measurement, 475 nm) in the presence of organic solvent	Isopropanol	10%	100	97	%	50 mM potassium phosphate buffer	7	25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	70%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	61	27	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	61	57	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	61	61	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	70%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	60%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	50%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	40%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	30%	61	21	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	20%	61	37	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Methanol	10%	61	48	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	70%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	60%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	50%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	40%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	30%	61	0	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	20%	61	26	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)
Stability - Incubation	Activity after incubation (17 hours at 30°C), measured by absorance spectrophotometry (dopaquinone spontaneous cyclization product, dopachrome, absorbance measurement, 475 nm) in the presence of organic solvent	Ethanol	10%	61	37	%	Incubation: 50 mM potassium phosphate buffer, Assay: 50 mM potassium phosphate buffer	7 buffer, 7 assay	Incubation: 30°C, Assay: 25°C	1 mM L-DOPA (L-3,4-Dihydroxyphenylalanine)	Dopaquinone	—	—	Water-incubated control (in %) \| Actvity measured in water before and after incubation, activity measured in the presence of organic solvent before and after incubation (mean of 3 measurements provided)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation