Xanthine oxidase from Bos taurus (Bovine)

Enzyme Description

Species

Extremophile

EC Number

1.17.3.2 ↗

Sequence

Length: 1332 amino acids

MTADELVFFVNGKKVVEKNADPETTLLAYLRRKLGLRGTKLGCGEGGCGACTVMLSKYDRLQDKIIHFSANACLAPICTLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTVEEIEDAFQGNLCRCTGYRPILQGFRTFAKNGGCCGGNGNNPNCCMNQKKDHTVTLSPSLFNPEEFMPLDPTQEPIFPPELLRLKDVPPKQLRFEGERVTWIQASTLKELLDLKAQHPEAKLVVGNTEIGIEMKFKNQLFPMIICPAWIPELNAVEHGPEGISFGAACALSSVEKTLLEAVAKLPTQKTEVFRGVLEQLRWFAGKQVKSVASLGGNIITASPISDLNPVFMASGTKLTIVSRGTRRTVPMDHTFFPSYRKTLLGPEEILLSIEIPYSREDEFFSAFKQASRREDDIAKVTCGMRVLFQPGSMQVKELALCYGGMADRTISALKTTQKQLSKFWNEKLLQDVCAGLAEELSLSPDAPGGMIEFRRTLTLSFFFKFYLTVLKKLGKDSKDKCGKLDPTYTSATLLFQKDPPANIQLFQEVPNGQSKEDTVGRPLPHLAAAMQASGEAVYCDDIPRYENELFLRLVTSTRAHAKIKSIDVSEAQKVPGFVCFLSADDIPGSNETGLFNDETVFAKDTVTCVGHIIGAVVADTPEHAERAAHVVKVTYEDLPAIITIEDAIKNNSFYGSELKIEKGDLKKGFSEADNVVSGELYIGGQDHFYLETHCTIAIPKGEEGEMELFVSTQNAMKTQSFVAKMLGVPVNRILVRVKRMGGGFGGKETRSTLVSVAVALAAYKTGHPVRCMLDRNEDMLITGGRHPFLARYKVGFMKTGTIVALEVDHYSNAGNSRDLSHSIMERALFHMDNCYKIPNIRGTGRLCKTNLSSNTAFRGFGGPQALFIAENWMSEVAVTCGLPAEEVRWKNMYKEGDLTHFNQRLEGFSVPRCWDECLKSSQYYARKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALIHVYTDGSVLVSHGGTEMGQGLHTKMVQVASKALKIPISKIYISETSTNTVPNSSPTAASVSTDIYGQAVYEACQTILKRLEPFKKKNPDGSWEDWVMAAYQDRVSLSTTGFYRTPNLGYSFETNSGNAFHYFTYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPTEFRVSLLRDCPNKKAIYASKAVGEPPLFLGASVFFAIKDAIRAARAQHTNNNTKELFRLDSPATPEKIRNACVDKFTTLCVTGAPGNCKPWSLRV

M. R. Rashidi et al. (2009) — Catalytic activity and stability of xanthine oxidase in aqueous-organic mixtures

Biochemistry (Moscow) · doi:10.1134/s0006297909010155 ↗ · Activity - Classical Stability - C50

153 measurements ▼

Database ID

UniProt: P80457 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Commercially purchased

Experimental Data (153 measurements)

153 measurements — page 7 of 8

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	7.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	5.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	6.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	13.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	10.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	9.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	8.5	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	10.7	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	15.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	17.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	15.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	6.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	14.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.7	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.4	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.4	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))

Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2.5%	100.0	76.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5%	100.0	43.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	7.5%	100.0	22.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100.0	11.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	12.5%	100.0	5.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	15%	100.0	3.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	17.5%	100.0	1.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	22.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	25%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	27.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2.5%	100.0	93.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100.0	81.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	7.5%	100.0	72.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100.0	62.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	12.5%	100.0	49.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	41.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	17.5%	100.0	30.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100.0	24.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	22.5%	100.0	20.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	25%	100.0	12.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	27.5%	100.0	9.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100.0	5.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	2.5%	100.0	90.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	5%	100.0	68.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	7.5%	100.0	49.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	10%	100.0	39.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	12.5%	100.0	19.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	15%	100.0	9.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	17.5%	100.0	6.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	20%	100.0	3.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	22.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	25%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	27.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	30%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	2.5%	100.0	98.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	5%	100.0	81.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	7.5%	100.0	60.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	10%	100.0	49.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	12.5%	100.0	43.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	15%	100.0	35.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	17.5%	100.0	28.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	20%	100.0	18.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	22.5%	100.0	13.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	25%	100.0	9.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	27.5%	100.0	6.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	30%	100.0	3.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	2.5%	100.0	81.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	5%	100.0	77.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	7.5%	100.0	67.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	10%	100.0	60.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	12.5%	100.0	56.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	15%	100.0	49.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	17.5%	100.0	44.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	20%	100.0	34.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	22.5%	100.0	33.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	25%	100.0	26.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	27.5%	100.0	18.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	30%	100.0	9.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	2.5%	100.0	60.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	5%	100.0	21.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	7.5%	100.0	7.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	10%	100.0	2.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	12.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	15%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	17.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	20%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	22.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	25%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	27.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	30%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	2.5%	100.0	14.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	5%	100.0	4.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	7.5%	100.0	2.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	10%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	12.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	15%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	17.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	20%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	22.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	25%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	27.5%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	30%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	2.5%	100.0	92.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	5%	100.0	75.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	7.5%	100.0	70.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	54.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	12.5%	100.0	45.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	32.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	17.5%	100.0	26.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	20%	100.0	14.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	22.5%	100.0	9.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	25%	100.0	4.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	27.5%	100.0	2.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	0.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	2.5%	100.0	98.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	5%	100.0	87.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	7.5%	100.0	80.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	10%	100.0	66.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	12.5%	100.0	61.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	15%	100.0	50.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	17.5%	100.0	37.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	20%	100.0	30.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	22.5%	100.0	16.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	25%	100.0	11.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	27.5%	100.0	8.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	30%	100.0	1.0	%	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Classical aqueous control (in %)
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	7.5	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	4.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	4.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	3.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	—	—	2.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	12.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	15.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	12.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	2.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Dimethylformamide (DMF)	—	—	12.4	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	11.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	8.5	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	7.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	5.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1-Propanol	—	—	6.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	13.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	10.6	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	9.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	8.5	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Isopropanol	—	—	10.7	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	15.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	17.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	15.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	6.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	1,4-Dioxane	—	—	14.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.7	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.4	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Pyridine	—	—	3.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.4	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.3	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Methanol	—	—	1.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	—	—	11.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	—	—	11.2	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	—	—	10.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	—	—	8.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Acetonitrile	—	—	8.0	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 27°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	—	—	18.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	27°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 32°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	—	—	15.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	32°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 37°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	—	—	14.9	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	37°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 42°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	—	—	13.8	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	42°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))
Stability - C50	Volume fraction of organic solvent for which half of the activity is lost at 50°C, measured by absorbance spectrophotometry (uric acid absorbance measurement, 295 nm) in the presence of organic solvent	Ethanol	—	—	8.1	% (v/v)	67 mM phosphate buffer, 0.1 M EDTA	7	50°C	20 µM Xanthine	Uric Acid	—	—	Not applicable control (in % (v/v))

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — C₅₀

One bar per measurement. Colour = solvent, shade = temperature. Hover for details.

Structure

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