Luciferase from Oplophorus gracilirostris (luminous shrimp)

Enzyme Description

Species

Oplophorus gracilirostris (luminous shrimp) ↗

Extremophile

EC Number

1.13.12.13 ↗

Sequence

Length: 169 amino acids

FTLADFVGDWQQTAGYNQDQVLEQGGLSSLFQALGVSVTPIQKVVLSGENGLKADIHVIIPYEGLSGFQMGLIEMIFKVVYPVDDHHFKIILHYGTLVIDGVTPNMIDYFGRPYPGIAVFDGKQITVTGTLWNGNKIYDERLINPDGSLLFRVTINGVTGWRLCENILA

Satoshi Inouye et al. (2007) — Overexpression, purification and characterization of the catalytic component of Oplophorus luciferase in the deep-sea shrimp, Oplophorus gracilirostris

Protein Expression and Purification · doi:10.1016/j.pep.2007.08.002 ↗ · Activity - Classical

63 measurements ▼

Database ID

UniProt: Q9GV45 ↗

Sequence Annotation

Inferred - from protein name (27 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (63 measurements)

63 measurements — page 4 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	15%	100	32	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	20%	100	9	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2) , coelenteramide , photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	1%	100	124	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide , Carbon Dioxide (CO2) , photon	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	4%	100	31	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	3%	100	167	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	5%	100	293	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	10%	100	469	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	15%	100	333	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	20%	100	100	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	30%	100	9	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	1%	100	162	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	2%	100	176	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	3%	100	260	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	5%	100	318	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	10%	100	216	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	15%	100	49	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	0.5%	100	100	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	1%	100	209	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	2%	100	327	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	2%	100	160	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	5%	100	7	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	10%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	30%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	50%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	100%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	1%	100	322	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	3%	100	491	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	5%	100	282	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	5%	100	256	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	10%	100	173	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	15%	100	16	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	5%	100	173	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	10%	100	247	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	15%	100	300	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	20%	100	120	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	10%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	2%	100	84	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	3%	100	84	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	5%	100	106	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	10%	100	90	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	15%	100	31	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	1%	100	93	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	3%	100	77	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	5%	100	85	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	10%	100	19	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Ethanol	15%	100	4	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	1%	100	81	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	2%	100	30	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	3%	100	8	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	4%	100	4	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	5%	100	1	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	1%	100	88	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	15%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	30%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	1-Butanol	50%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	1%	100	98	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	3%	100	27	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	5%	100	7	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	2-Mercaptoethanol	10%	100	0	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	5%	100	65	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Acetone	10%	100	10	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	5%	100	89	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	10%	100	72	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	15%	100	32	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Dimethyl Sulfoxide (DMSO)	20%	100	9	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Coelenterazine, ? mM O₂	Carbon Dioxide (CO2), coelenteramide, photon	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (luminescence measurement, 454 nm)	Methanol	1%	100	124	%	30 mM Tris-HCl buffer, 10 mM EDTA	7.6	25°C	1 µg/µL Bis-coelenterazine	Bis-coelenteramide, Carbon Dioxide (CO2), photon	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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