Serine protease from Natrialba magadii

Enzyme Description

Species

Natrialba magadii ATCC 43099 ↗

Extremophile

Yes "haloalkaliphilic" organism

EC Number

3.4.21.- ↗

Sequence

Length: 541 amino acids

MTRDTNSNVGRRSVLKAASALGAFLGLGGVASATPGREPGPKKDEIIVGVSERVSSTEATVESKIPTNAEIVHTNETLGYVAVKFPSNAAEQARENFKRNVLQEDDIEYAEDNATYETLEVPNDPMYGQQYAPQQVNCEGAWAETYGDDDVVISVVDQGIQYDHENLAENMDGSVSDYGYDFVDDDGDPYPVSAGENHGTHVGGIAAGGTNNDTGHAGISNCSMLSARALGDGGGGSLSDIADAIQWSADQGADIINMSLGGGGFSETLDNACQYAYDEGTLLVAAAGNDHGGSVSYPAAYDSVMAVSSLDEGETLSSFSNVGPEIELAAPGGNVLSAVNWDDYDSLSGTSMASPVAAGVAGLALSAHPGLSNDELRDHLHDTAVDIGLSDDEQGYGRVDAELAVTTDPDNGDDDDDDDDDEDDPGDGECGDETNTATADGELSGGWGGNPSDTYSYELSTDNPCHATVTLDGPSSGATFDLFLTLDGRTPTTSDYDRRSYNWGADEEIEVDLDGDEELGILVDRYDGSGSYTLTIEELGS

Diego M. Ruiz et al. (2006) — Effect of organic solvents on the activity and stability of an extracellular protease secreted by the haloalkaliphilic archaeon Natrialba magadii

Journal of Industrial Microbiology and Biotechnology · doi:10.1007/s10295-006-0174-4 ↗ · Activity - Classical Stability - Incubation

52 measurements ▼

Database ID

UniProt: Q5RLZ1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Purified, archaeon Natrialba magadii

Experimental Data (52 measurements)

52 measurements — page 1 of 3

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	83	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	15%	100	76	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	30%	26	13	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	26	2	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	26	72	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	30%	26	38	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Isopropanol	15%	26	0	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Ethanol	15%	26	1	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetonitrile	15%	26	0	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	15%	26	1	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	1,4-Dioxane	15%	26	5	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	15%	26	26	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	26	5	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	26	50	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	30%	100	20	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	15%	26	33	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	76	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	15%	100	44	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	1,4-Dioxane	15%	100	30	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	15%	100	22	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	83	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	15%	100	76	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	30%	26	13	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	26	2	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	26	72	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	30%	26	38	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Isopropanol	15%	26	0	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Ethanol	15%	26	1	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetonitrile	15%	26	0	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	15%	26	1	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	1,4-Dioxane	15%	26	5	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	15%	26	26	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	26	5	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	26	50	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	30%	100	20	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	15%	26	33	%	50 mM Tris-HCl, 0.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	76	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Propylene Glycol	15%	100	44	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	1,4-Dioxane	15%	100	30	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetone	15%	100	22	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Acetonitrile	15%	100	8	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Ethanol	15%	100	6	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Isopropanol	15%	100	2	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Glycerol	30%	100	54	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	48	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	22	%	50 mM Tris-HCl, 1.5M NaCl	8	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Glycerol	15%	0	14	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	0	48	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethylformamide (DMF)	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Propylene Glycol	15%	0	14	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	1,4-Dioxane	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Acetone	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Acetonitrile	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Ethanol	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Isopropanol	15%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Glycerol	30%	0	89	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	0	56	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethylformamide (DMF)	30%	0	0	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Propylene Glycol	30%	0	55	%	Incubation: 50 mM Tris-HCl, with 0.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Propylene Glycol	30%	100	102	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100	57	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	111	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Glycerol	30%	100	108	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Isopropanol	15%	100	23	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Ethanol	15%	100	52	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Acetonitrile	15%	100	2	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Acetone	15%	100	59	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	1,4-Dioxane	15%	100	93	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Propylene Glycol	15%	100	130	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethylformamide (DMF)	15%	100	73	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	15%	100	99	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")
Stability - Incubation	Activity after incubation (24 hours at 30°C) measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 335 nm) in aqueous phase	Glycerol	15%	100	107	%	Incubation: 50 mM Tris-HCl, with 1.5M NaCl, Assay: 50 mM Tris-HCl, 1.5M NaCl	Incubation: 8, Assay: 8	Incubation: 45°C, Assay: 45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase (1.5 M NaCl) \| Clear about diluted conditions in assay ("Final solvent concentration below 6%")

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation